Immune responses of Asian sea bass, Lates calcarifer Bloch, against an inactivated betanodavirus vaccine
Asian sea bass, Lates calcarifer (Bloch), exhibited strong immune responses against a single injection of the formalin-inactivated red-spotted grouper nervous necrosis virus (RGNNV), a betanodavirus originally isolated in Japan. Fish produced neutralizing antibodies at high titre levels from days 10 (mean titre 1:480) to 116 (1:1280), with the highest titre at day 60 post-vaccination (1:4480). When fish were challenged with the homologous RGNNV at day 54 post-vaccination, there were no mortalities in both the vaccinated and unvaccinated control fish. However, a rapid clearance of the virus was observed in the brains and kidneys of vaccinated fish, followed by a significant increase in neutralizing-antibody titres. Furthermore, the vaccine-induced antibodies potently neutralized Philippine betanodavirus isolates (RGNNV) in a cross-neutralization assay. The present results indicate the potential of the formalin-inactivated RGNNV vaccine against viral nervous necrosis (VNN) of Asian seabass.
Document Type: Research Article
Affiliations: 1: Southeast Asian Fisheries Development Center, Tigbauan, Iloilo, Philippines 2: National Research Institute of Aquaculture, Fisheries Research Agency, Saiki, Oita, Japan 3: Ehime Fisheries Research Center, Uwajima, Ehime, Japan 4: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan
Publication date: 2009-05-01