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Rapid development of polyclonal antisera against infectious salmon anaemia virus and its optimization and application as a diagnostic tool

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Abstract:

Abstract

Infectious salmon anaemia is an important disease of Atlantic salmon. One of the current methods of diagnosis is the indirect fluorescent antibody test (IFAT), using a monoclonal antibody specific to the haemagglutinin of the virus. The conformationally dependent nature of this antibody could be a drawback in its usefulness in other tests. This study describes the development and optimization of a polyclonal antiserum against infectious salmon anaemia virus, including a method of separating virus from cell culture components within culture supernatant. The antiserum was subsequently optimized for use in a variety of immunological diagnostic tests, including IFAT and an alkaline phosphatase-based immunoassay, and Western blot.

Keywords: diagnostic tests; enzyme immunoassay; indirect fluorescent antibody test; infectious salmon anaemia virus; monoclonal antibody; polyclonal antisera

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1365-2761.2006.00720.x

Publication date: May 1, 2006

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