Skip to main content

Rapid detection of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV), the pathogenic agents of white tail disease of Macrobrachium rosenbergii (De Man), by loop-mediated isothermal amplification

Buy Article:

$43.00 plus tax (Refund Policy)


A loop-mediated isothermal amplification (LAMP) procedure is described for rapid diagnosis of white tail disease, a viral disease caused by Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV), in the giant freshwater prawn, Macrobrachium rosenbergii. This method was more sensitive than conventional RT-PCR for detecting the two viruses. A set of four primers, two outer and two inner, were designed for MrNV detection. An additional pair of loop primers was also used in an accelerated LAMP reaction for detection of XSV. Time and temperature conditions were optimized for detection of the two viruses. The LAMP reaction is highly suited for disease diagnosis in developing countries as amplification of DNA can be detected without the use of agarose gel electrophoresis, by the production of whitish precipitate of magnesium pyrophosphate as a by-product.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: M. rosenbergii nodavirus (MrNV); Macrobrachium rosenbergii; diagnosis; extra small virus (XSV); loop-mediated isothermal amplification (LAMP); white tail disease

Document Type: Research Article

Affiliations: 1:  Department of Aquaculture, College of Fisheries, Kerala Agricultural University, Cochin, India 2:  Pathogens and Immunity, UMR 5119 CNRS-UM2, Ecolag, Universit√© Montpellier II, Montpellier, France

Publication date: 2006-05-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more