Modulation of β-actin, insulin-like growth factor 1 and glucose-6-phosphate dehydrogenase gene expression during vibriosis of sea bream, Sparus (=Rhabdosargus) sarba Forsskål
Gene expression changes, related to growth regulation, were investigated in silver sea bream, Sparus (=Rhabdosargus) sarba, subjected to vibriosis. Using reverse transcriptase polymerase chain reaction assays, transcript levels of β-actin, insulin-like growth factor 1 (IGF-1) and glucose-6-phosphate dehydrogenase (G6PDH) were analysed. A tissue-specific response for β-actin was found, as transcript levels in the kidney increased during early- and mid-stage vibriosis, but remained unchanged in liver tissue. The gene transcript levels of IGF-1 were found to decrease in both liver and kidney from an early stage of vibriosis. The gene transcript levels of G6PDH increased at early and mid stages of vibriosis in liver and kidney before decreasing during the late stage of vibriosis. Further studies on G6PDH demonstrated that chemical inhibition of G6PDH in silver sea bream fibroblasts resulted in increased cell death upon exposure to hydrogen peroxide. The results from the present study demonstrate the complexity of growth-related gene expression events that occur in fish tissues during disease.