IDENTIFICATION AND CHARACTERIZATION OF MATRIX METALLOPROTEINASES FROM THE SARCOPLASMIC FRACTION OF COMMON CARP (CYPRINUS CARPIO) DARK MUSCLE

Authors: WU, JIU-LIN¹; CAO, MIN-JIE¹; FU, XIAO-PING¹; LIU, GUANG-MING¹; ZHOU, LI-GEN²; HARA, KENJI³; SU, WEN-JIN¹

Source: Journal of Food Biochemistry, Volume 33, Number 5, October 2009 , pp. 745-762(18)

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Abstract:

Two gelatin hydrolyzing proteinases in the sarcoplasmic fraction of common carp muscle were detected using gelatin zymography. A comparative study shows that the gelatin hydrolyzing activity in dark muscle is obviously higher than that in white muscle. The enzymes can transform to their active forms after treated by aminophenylmercuric acetate, an activator of matrix metalloproteinases. Optimum pH and temperature of these enzymes were around 8.0 and 40C using gelatin as substrate. Metalloproteinase inhibitors (ethylenediaminetetraacetic acid and ethylene glycol-bis (2-aminoethylother)-N, N, N′, N′-tetraacetic acid) completely suppressed the activities and 1,10-phenanthroline also showed great inhibitory effects. However, other proteinase inhibitors, such as soybean trypsin inhibitor, benzamidine, E-64 and pepstatin A, did not show any effect. Metal ions Ca²⁺ and Zn²⁺ are essential for these gelatinolytic activities. All these facts indicate that these proteinases are matrix metalloproteinases. Furthermore, these enzymes hydrolyze collagen effectively and maybe thus proposed to be responsible for the tenderization of fish muscle during the postmortem stage.

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1745-4514.2009.00248.x

Affiliations: 1: College of Biological EngineeringThe Key Laboratory of Science and Technology for Aquaculture and Food Safety, Jimei UniversityJimei, Xiamen 361021, China 2: Institute of Food ProcessingZhejiang Academy of Agriculture SciencesHangzhou 310021, China 3: Faculty of FisheriesNagasaki UniversityNagasaki 852-8521, Japan

Publication date: 2009-10-01

IDENTIFICATION AND CHARACTERIZATION OF MATRIX METALLOPROTEINASES FROM THE SARCOPLASMIC FRACTION OF COMMON CARP (CYPRINUS CARPIO) DARK MUSCLE

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