Authors: SHUANG, DING¹; JIANG-KE, YANG²; YUN-JUN, YAN

Source: Journal of Food Biochemistry, Volume 33, Number 3, June 2009 , pp. 442-452(11)

Publisher: Wiley-Blackwell

Abstract:

The condition optimization of lipase-catalyzed acidolysis of soybean oil was conducted by using the response surface methodology (RSM). The results showed that when decision coefficient was 98.34%, the optimal conditions for highest caprylic acid (CA) incorporation was: reaction temperature 35.8C, lipase dosage 11.9% (w/w of substrate), substrate ratio 5.7 (oil/CA, mol/mol), water content 15.4% (w/w of enzyme) and reaction time 20.4 h, and under which conditions, the actually measured incorporation of CA was up to 44.9 mol%, very close to the predicted value 45.7 mol%. This indicates that with RSM, we can effectively optimize the reaction conditions of lipase-catalyzed acidolysis of soybean oil to produce structured lipids. PRACTICAL APPLICATIONS

In digestion procedure, pancreatic and gastric lipases both have preference for ester bonds located at sn-1 and sn-3 position in triacylglycerols (TGs), and show higher activity towards short- and medium-chain fatty acids (FAs). Dietary TGs are hydrolyzed to sn-2 monoacylglycerols and FAs in the intestine by lipases. Therefore, long-chain polyunsaturated FAs located at sn-2 position may provide sn-2 monoacylglycerols, which are the raw materials in synthesis of TGs in mucosal cells if the dietary fats or oils are MLM- or SLS-type TGs (SLS or MLM are the short-chain or medium-chain FAs located at sn-1 and sn-3 position, while the long-chain FAs are located at sn-2). The short-chain or medium-chain FAs located at sn-1 and sn-3 position are absorbed by the portal vein and rapidly oxidized in the liver as quick source of energy instead of glucose.

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1745-4514.2009.00227.x

Affiliations: 1: Minnan Science and Technology InstituteFujian Normal UniversityNan'an, Fujian 362332, China 2: College of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074, China

Publication date: 2009-06-01

Related content

• In this: publication
• By this: publisher
• In this Subject: Agriculture/Food Sciences ,  Biochemistry
• By this author: SHUANG, DING ;  JIANG-KE, YANG ;  YUN-JUN, YAN

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers