Authors: GOMEZ, LEISSY¹; RAMIREZ, HECTOR L.¹; CABRERA, GUSTAVO²; SIMPSON, BENJAMIN K.³; VILLALONGA, REYNALDO

Source: Journal of Food Biochemistry, Volume 32, Number 2, April 2008 , pp. 264-277(14)

Publisher: Wiley-Blackwell

Abstract:

Baker yeast invertase, chemically modified with chitosan, was immobilized on hyaluronic-acid-coated chitin support via polyelectrolyte complex formation. The immobilized enzyme retained 80% of the initial invertase activity. The optimum temperature for sucrose hydrolysis was increased by 5C, and its thermostability was enhanced by about 10C after immobilization. The immobilized enzyme was stable against incubation in high-ionic-strength solutions, and was sixfold more resistant to thermal treatment at 65C than the native counterpart. The biocatalyst prepared retained 100% after 10 cycles of reuse as well as after 74 h of continuous sucrose hydrolysis in a packed bed reactor, respectively. The immobilized enzyme retained 69% of its activity after 50 days of storage at 37C. PRACTICAL APPLICATIONS

Invertase is widely used in the food industry as biocatalyst for the preparation of invert sugar syrups, a value-added product obtained from sucrose. The present article deals with the preparation of a highly stable invertase by modifying it with chitosan and then immobilizing it on hyaluronic-acid-coated chitin support. This new biocatalyst has potential application in the high-scale preparation of sucrose syrups, reducing industrial costs because of the excellent operational stability and reusability properties showed by the immobilized enzyme.

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1745-4514.2008.00170.x

Affiliations: 1: Center for Enzyme TechnologyUniversity of MatanzasMatanzas, C.P. 44740, Cuba 2: Facultad de Recursos NaturalesEscuela de Ingeniería AgrícolaUniversidad Católica de TemucoTemuco, Chile 3: Food Science and Agricultural Chemistry DepartmentMcGill University (Macdonald Campus)21,111 Lakeshore Road, Ste Anne de Bellevue, Québec, Canada H9X 3V9

Publication date: 2008-04-01

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