Effectiveness of TaqMan probes for detection of fish eggs and larvae in the stomach contents of a teleost predator

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Abstract:

Experiments were conducted on the ability of TaqMan molecular probes to detect plaice Pleuronectes platessa DNA from eggs, and cod Gadus morhua DNA from eggs and larvae following ingestion by a teleost predator, whiting Merlangius merlangus. Estimated half‐life detection rate (T50) for eggs was 31 h, and 26 h for larvae, with some positive detections occurring even after visual inspection indicated complete gut clearance. Because TaqMan probes are taxon specific, the results presented demonstrate that this technique can provide a means of rapid and unambiguous detection of predation by teleosts on fish eggs and larvae.

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1095-8649.2012.03298.x

Publication date: July 1, 2012

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