Successful extraction of DNA from archived alcohol-fixed white-eye fish specimens using an ancient DNA protocol

Authors: deBruyn, M.; Parenti, L. R.¹; Carvalho, G. R.²

Source: Journal of Fish Biology, Volume 78, Number 7, June 2011 , pp. 2074-2079(6)

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Abstract:

A protocol used routinely for rapid ancient DNA extraction was applied to fish tissue archived over 80 years ago. The method proved successful, whereas other extraction protocols failed. Researchers working on DNA from older archived fish samples are encouraged to continue to concentrate their efforts on `white-eye' specimens, which indicate an alcohol-based fixative and are thus likely to yield viable DNA.

Keywords: archived tissue; chloroform; formalin; museum specimen; PCR; phenol

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1095-8649.2011.02975.x

Affiliations: 1: Department of Vertebrate Zoology, P. O. Box 37012, MRC 159, National Museum of Natural History, Smithsonian Institution, Washington, DC 20013-7012, U.S.A. 2: Molecular Ecology and Fisheries Genetics Laboratory, Environment Centre for Wales, Biological Sciences, Bangor University, Deiniol Road, Bangor LL57 2UW, U.K.

Publication date: 2011-06-01