Discrimination of two picarel species Spicara flexuosa and Spicara maena (Pisces: Centracanthidae) based on mitochondrial DNA sequences
Abstract:A DNA methodology based on PCR and sequencing analysis of the mtDNA 16S rRNA gene was developed for the discrimination of picarel Spicara flexuosa and blotched picarel Spicara maena (Pisces: Centracanthidae). The molecular results indicated that there is a clear discrimination between the two species, as all the individuals of S. flexuosa revealed the same 16S rDNA haplotype while the S. maena haplotype differs in 15 distinct nucleotides.
Document Type: Research Article
Affiliations: 1: Alexander Technological Educational Institute of Thessaloniki, Department of Aquaculture & Fisheries Technology, P. O. Box 157, GR-63200, Nea Moudania, Greece 2: Technological Educational Institute of Messolonghi, Department of Aquaculture & Fisheries Management, GR-30200, Messolonghi, Greece
Publication date: January 1, 2011