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Discrimination of two picarel species Spicara flexuosa and Spicara maena (Pisces: Centracanthidae) based on mitochondrial DNA sequences

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Abstract:

A DNA methodology based on PCR and sequencing analysis of the mtDNA 16S rRNA gene was developed for the discrimination of picarel Spicara flexuosa and blotched picarel Spicara maena (Pisces: Centracanthidae). The molecular results indicated that there is a clear discrimination between the two species, as all the individuals of S. flexuosa revealed the same 16S rDNA haplotype while the S. maena haplotype differs in 15 distinct nucleotides.

Keywords: 16S rRNA; blotched picarel; genetic identification; picarel; sequencing

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1095-8649.2010.02858.x

Affiliations: 1: Alexander Technological Educational Institute of Thessaloniki, Department of Aquaculture & Fisheries Technology, P. O. Box 157, GR-63200, Nea Moudania, Greece 2: Technological Educational Institute of Messolonghi, Department of Aquaculture & Fisheries Management, GR-30200, Messolonghi, Greece

Publication date: January 1, 2011

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