Skip to main content

Derivation and characterization of embryonic stem-like cells of Indian major carp Catla catla

Buy Article:

$43.00 plus tax (Refund Policy)

Embryonic stem (ES)-like cells were derived from mid-blastula stage embryos of a freshwater fish, catla Catla catla, under feeder-free condition and designated as CCES cells. The conditioned media was optimized with 10% foetal bovine serum (FBS), fish embryo extract (FEE) having 100 µg ml−1 protein concentration, 15 ng ml−1 basic fibroblast growth factor (bFGF) and basic media containing Leibovitz-15, DMEM with 4·5 g l−1 glucose and Ham's F12 (LDF) in 2:1:1 ratio using a primary culture of CCES cells. Cells attached to gelatin-coated plates after 24 h of seeding and ES-like colonies were obtained at day 5 onwards. A stable cell culture was obtained after passage 10 and further maintained up to passage 44. These cells were characterized by their typical morphology, high alkaline phosphatase activity, positive expression of cell-surface antigen SSEA-1, transcription factor Oct4, germ cell marker vasa and consistent karyotype up to extended periods. The undifferentiated state was confirmed by their ability to form embryoid bodies and their differentiation potential.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: RT-PCR; bFGF; catla; immunocytochemistry; stem cell

Document Type: Regular Paper

Affiliations: 1: Division of Aquaculture Production and Environment, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar 751002, India 2: Post-Graduate Department of Zoology, Utkal University, Bhubaneswar 751004, India 3: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar 751002, Orissa, India

Publication date: 2010-10-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more