Acute toxic effects of 8-epidiosbulbin E, a 19-norclerodane diterpene from yam Dioscorea antaly, on medaka Oryzias latipes embryos

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Abstract:

The fractionation of an aqueous extract of yam Dioscorea antaly from Madagascar led to the isolation of terpenoids and flavonoids. Compounds were identified on the basis of modern mass spectrometry and two-dimensional nuclear magnetic resonance (2D-NMR). Toxicological effects of the most abundant isolated compound, 8-epidiosbulbin E were studied on medaka Oryzias latipes embryo-larval development. The lethal concentration (killing 50%; LC50) to embryos treated 24 h before hatching and for 3 days after hatching was estimated to be 0·56 mg ml−1 (P< 0·05). No mortality was observed with O. latipes larvae exposed after hatching until day 4. Anatomo-pathological studies of embryos exposed to 0·56 mg ml−1 showed development anomalies of the central nervous system, liver, muscle and intestine. The present data thus extend the model of O. latipes embryos as a useful animal model to analyse the effects of food toxins.

Keywords: fish eggs; hepatotoxicity; neurotoxicity; plant toxins

Document Type: Regular Paper

DOI: http://dx.doi.org/10.1111/j.1095-8649.2010.02720.x

Affiliations: 1: Laboratoire de Microbiologie et de Biologie Moléculaire, Centre National des Sciences et Techniques Nucléaires, Sidi Thabet, 2020 Tunis, Tunisia 2: FRE 3206 CNRS Molécules de communication et adaptation des micro-organismes, Muséum National d’Histoire Naturelle, 12 rue Buffon, F-75231 Paris Cedex 05, France 3: Département de Biochimie fondamentale et appliquée, Faculté des Sciences de l’Université d’Antananarivo, B.P. 906, 101, Antananarivo, Madagascar

Publication date: September 1, 2010

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