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Plasma cortisol and 11-ketotestosterone enzyme immunoassay (EIA) kit validation for three fish species: the orange clownfish Amphiprion percula, the orangefin anemonefish Amphiprion chrysopterus and the blacktip reef shark Carcharhinus melanopterus

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Abstract:

Commercially available enzyme immunoassay (EIA) kits were validated for measuring steroid hormone concentrations in blood plasma from three fish species: the orange clownfish Amphiprion percula, the orangefin anemonefish Amphiprion chrysopterus and the blacktip reef shark Carcharhinus melanopterus. A minimum of 5 µl plasma was required to estimate hormone concentrations with both kits. These EIA kits are a simple method requiring minimal equipment, for measuring hormone profiles under field conditions.

Keywords: ELISA; elasmobranch; haematocrit; hormone; steroids; teleost

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1095-8649.2010.02693.x

Affiliations: USR 3278 CNRS-EPHE, CRIOBE-CBETM, Université de Perpignan, 66860 Perpignan Cedex, France

Publication date: August 1, 2010

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