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Synthesis of 17,20,21-trihydroxypregn-4-en-3-one by ovaries of reproductively mature Atlantic cod Gadus morhua

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Abstract:

Atlantic cod Gadus morhua ovaries were incubated in vitro with tritiated 17-hydroxypregn-4-ene-3,20-dione (17-P) to determine whether 17,20-dihydroxypregn-4-en-3-one (17,20-P) or 17,20, 21-trihydroxypregn-4-en-3-one (17,20,21-P), or both, were more likely to be the steroid responsible for inducing oocyte final maturation (i.e. resumption of meiosis). Only 17,20,21-P was produced, in addition to 11-deoxycortisol (17,21-P), which is intermediate between 17-P and 17,20,21-P. Also, the 5-reduced forms of 17-P, 17,21-P and 17,20,21-P were all found. Some sulphation of 21-hydroxylated steroids was demonstrated. The ability of female G. morhua to make 17,20,21-P but not 17,20-P was confirmed by radioimmunoassay of plasma samples from spawning fish. Although small amounts of 17,20-P immunoreactivity were detected in a few plasma samples, this was shown, by thin-layer chromatography, to be mostly due to cross-reaction with other unidentified compounds. The evidence strongly suggests that 17,20,21-P is more likely than 17,20-P to be the maturation-inducing steroid in G. morhua.

Keywords: 11-deoxycortisol; maturation-inducing steroid (MIS)

Document Type: Regular Paper

DOI: http://dx.doi.org/10.1111/j.1095-8649.2010.02655.x

Affiliations: Centre for Environment, Fisheries and Aquaculture Science, Barrack Road, Weymouth DT4 9PB, U.K.

Publication date: July 1, 2010

bsc/jfb/2010/00000077/00000001/art00003
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