Skip to main content

Molecular identification and expression analysis of Toll-like receptor 3 in common carp Cyprinus carpio

Buy Article:

$43.00 plus tax (Refund Policy)

The Toll-like receptor (TLR) family is an evolutionarily conserved component of the innate immune system that responds to specific pathogen-associated molecular patterns (PAMP) during infections. TLR3 is a putative pattern recognition receptor (PRR) for dsRNA, a common by-product of viral replication. This study reports the identification and characterization of a full-length orthologue of TLR3 (CcTLR3) in the common carp Cyprinus carpio. It is 2899 base pairs (bp) encoding 904 amino acid residues with a calculated molecular mass of 102 744 Da and an estimated isoelectric point of 6·89. The deduced amino acid sequence is characterized by a signal peptide, 14 leucine-rich repeat (LRR) motifs, a transmembrane domain and a Toll–interleukin (IL)-1 receptor (TIR) domain. Real-time polymerase chain reaction (RT-PCR) analysis indicated that CcTLR3 mRNA transcripts could be detected in a wide range of tissues. Although C. carpio is resistant to grass carp Ctenopharyngodon idella reovirus (GCRV), after injection with GCRV, CcTLR3 expression was downregulated from 24 h post-injection (P < 0·05) and returned to control levels at 72 h post-injection (P > 0·05). Antiviral effector Mx mRNA expression, however, was upregulated from 24 h post-injection (P < 0·05) and recovered to control levels at 96 h post-injection (P > 0·05). The results provide a better understanding of the anti-GCRV mechanism and antiviral immune responses in fish, and help to develop suitable strategies to reduce viral diseases in fish.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Toll-like receptor 3; common carp Cyprinus carpio; gene cloning; mRNA expression

Document Type: Regular Paper

Affiliations: Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China

Publication date: 2010-05-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more