An osmotic induction method for externally marking saltwater fishes, Stigmatopora argus and Stigmatopora nigra, with calcein

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Abstract:

Calcein marking via osmotic induction and overnight immersion was compared in saltwater fishes. Immersion in a salt solution for 10 min followed by 30 min in a 500 mg l−1 calcein solution produced the highest fluorescent mark in 40 min with no effect on survival.

Keywords: chemical marking; fluorescence intensity; tag

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1095-8649.2010.02551.x

Affiliations: Department of Zoology, University of Melbourne, Parkville, Victoria 3010, Australia

Publication date: March 1, 2010

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