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In vitro expansion of autonomously contracting, cardiomyogenic structures from rainbow trout Oncorhynchus mykiss

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In primary cell preparations from larvae of rainbow trout Oncorhynchus mykiss, the formation of autonomously contracting cell aggregates was observed after 7 days. These contracting elements could be propagated and some aggregates were maintained over a period of 35 days. Electron microscopical and immunocytochemical examination revealed the presence of cardiomyocytes.

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Keywords: Oncorhynchus mykiss; cardiac regeneration; fish heart; immunohistochemistry; primary cell culture; rainbow trout

Document Type: Research Article

Affiliations: 1: Fraunhofer Institute for Marine Biotechnology, Fraunhofer Society, Paul Ehrlich Str. 1-3, 23562, Lübeck, Germany 2: University of Lübeck, Institute of Anatomy, Ratzeburger Allee 160, 23562, Lübeck, Germany 3: Research Institute for Agriculture and Fishery, Mecklenburg-Vorpommern, Südstr. 8, 18357, Born/Darß, Germany

Publication date: 2010-02-01

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