No sex-specific markers detected in bluegill sunfish Lepomis macrochirus by AFLP

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Abstract:

The amplified fragment-length polymorphism (AFLP) technique was used to identify sex-specific markers in bluegill sunfish. A total of 12 835 loci were produced by 256 primer combinations, of which nine (0ยท73‰) exhibited presumed sex-associated amplifications in the pooled samples; however, none of which revealed sex specificity upon individual evaluation.

Keywords: AFLP; Lepomis macrochirus; sex-specific marker

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1095-8649.2009.02508.x

Affiliations: 1: Aquaculture Genetics and Breeding Laboratory, Ohio State University Aquaculture Research and Development Integration Program, 1864 Shyville Road, Piketon, OH 45661, U.S.A. 2: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Fishery, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China

Publication date: February 1, 2010

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