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Factors affecting mark quality of alizarin complexone-stained otoliths in juvenile black bream Acanthopagrus butcheri and a prescription for dosage

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The relationships between otolith score, a function of increasing stain visibility, and the ratio of alizarin complexone (mg) to fish biomass (g) for 0·1, 0·6 and 7 g black bream Acanthopagrus butcheri show that acceptable marks are produced in all size classes using a stain-to-biomass ratio of 3·3 mg g−1 following an immersion period of 24 h.
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Keywords: chemical-staining techniques; fish identification; fish otolith marking

Document Type: Research Article

Affiliations: 1: Aquaculture Development Unit, WA Maritime Training Centre, 1 Fleet Street, Fremantle, Western Australia 6160, Australia 2: Centre for Fish and Fisheries Research, Murdoch University, Murdoch, Western Australia 6150, Australia

Publication date: 2009-10-01

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