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Identification of weakfish Cynoscion (Gill) in the Bay of Panama with RFLP markers

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Abstract:

A molecular approach, polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP), was developed to identify the planktonic larvae of Cynoscion species. Species-specific mitochondrial DNA markers were developed using three restriction endonucleases (DdeI, HaeIII and HinfI). These markers permitted the accurate discrimination of the five Cynoscion species in the Bay of Panama.

Keywords: PCR-RFLP; cytochrome b; fish larvae; marine fish; mitochondrial DNA; species identification

Document Type: Research Article

DOI: https://doi.org/10.1111/j.1095-8649.2009.02356.x

Affiliations: 1: Departamento de Ecología e Hidrología, Facultad de Biología, Universidad de Murcia, Campus de Espinardo, 30100 Murcia, Spain 2: Departamento de Biología Marina y Limnología, Universidad de Panamá, Estafeta Universitaria, Panamá

Publication date: 2009-10-01

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