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31P nuclear magnetic resonance measurements of phosphate metabolites and intracellular pH in turbot Psetta maxima red blood cells using a novel flow method

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Abstract:

A method for oxygenating and mixing suspensions of turbot Psetta maxima red blood cells (RBC) was tested in 31P nuclear magnetic resonance (NMR) spectroscopy. In normoxia, the levels of inorganic phosphate (Pi) and nucleoside triphosphates (NTP) were stable up to 140 min and intracellular pH (pHi) was maintained and decreased oxygen partial pressure (PO 2) from 30 to 15 and 600 Pa produced a significant fall in the intensity of NTP resonance, balanced by an increase in the Pi signal. Treatment of RBC with 0· 5 M isoproterenol during hypoxia exposure did not affect the pattern of changes in NTP or pHi induced by hypoxia and the effect was manifest only on Pi levels.

Keywords: energetic status; fish; flow-cell apparatus

Document Type: Research Article

DOI: https://doi.org/10.1111/j.1095-8649.2009.02351.x

Affiliations: 1: Laboratoire de Physiologie et Biologie Cellulaire, UFR. Sciences et Techniques, Université de Brest, Brest, France and 2: Service de RMN, Université de Brest, Brest, France

Publication date: 2009-08-01

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