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In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio

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Abstract:

The authors show that co-injection at the one-cell stage of mRNA encoding a nuclear-targeted meganuclease I-SceI together with expression cassettes flanked by cognate restriction sites results in efficient stable transgenesis in zebrafish Danio rerio.

Keywords: Gal4; I-SceI; meganuclease; transgenesis; zebrafish

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1095-8649.2008.02075.x

Affiliations: Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Developmental Genetics, Ingolstädter Landstraße 1, 85764 Neuherberg-Munich, Germany

Publication date: February 1, 2009

bsc/jfb/2009/00000074/00000002/art00007
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