In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio
Abstract:The authors show that co-injection at the one-cell stage of mRNA encoding a nuclear-targeted meganuclease I-SceI together with expression cassettes flanked by cognate restriction sites results in efficient stable transgenesis in zebrafish Danio rerio.
Document Type: Research Article
Affiliations: Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Developmental Genetics, Ingolstädter Landstraße 1, 85764 Neuherberg-Munich, Germany
Publication date: 2009-02-01