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Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus

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Abstract:

Chinese sturgeon Acipenser sinensis, a cartilaginous ganoid, is a ‘living fossil’ on a deeply isolated evolutionary branch. A cell line was established from Chinese sturgeon tail-fin tissue (CSTF) . These epithelial CSTF cells grew well in Dulbecco’s modified Eagle’s medium at 25° C. Karyotypic analysis revealed a normal diploid karyotype with 2n= 264 and large numbers of punctate chromosomes. A strain of frog iridoviruses [Rana grylio virus (RGV)] was used to test the susceptibility of this cell line to infection. Infection was confirmed by cytopathic effect, immunofluorescence and electron-microscope observations, which detected the viral antigens or particles in the cytoplasm of RGV-infected cells. Molecular analysis further suggested that c. 550 bp DNA fragment could be cloned from the RGV-infected CSTF cells’ DNA with major capsid protein gene polymerase chain reaction primers. Furthermore, after transfection with pEGFP vector DNA, the CSTF cell line produced significant fluorescent signals indicating its utility in exogenous studies.

Keywords: chromosome; cytopathic effect; electron microscope; freshwater fish; immunofluorescence; karyotype

Document Type: Regular Paper

DOI: http://dx.doi.org/10.1111/j.1095-8649.2008.02076.x

Affiliations: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

Publication date: December 1, 2008

bsc/jfb/2008/00000073/00000008/art00014
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