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Genomic resources and microarrays for the common carp Cyprinus carpio L.

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Abstract:

The common carp is an important fish species satisfying ornamental, food and recreational fisheries’ needs worldwide, but in common with other cyprinid fishes, it is particularly renowned for its environmental tolerance. Investigating the mechanistic basis of growth, disease and environmental tolerance is greatly enhanced by access to a comprehensive list of gene sequences and post-genomic technologies. The current status of genomic resources is described for this species including 40 k cDNA clone collections, their associated expressed sequence tags (ESTs) and a developing series of 13 k–26 k cDNA microarrays fabricated from amplicons. The arrays have been directed at questions of response to environmental stress (cold and hypoxia), viral and bacterial disease and ectoparasite infection. Consequently, clones from a wide range of tissues were prepared. The authors discuss how these resources were generated and their application. Evidence is presented supporting that the carp microarray may also be useful as a heterologous set of probes in studies of other fish species.

Keywords: cDNA; expressed sequence tags; microarray; transcripts

Document Type: Regular Paper

DOI: http://dx.doi.org/10.1111/j.1095-8649.2008.01875.x

Affiliations: 1: Laboratory for Environmental Gene Regulation, University of Liverpool, Crown Street, Liverpool L69 7ZB, U.K. 2: Research Institute for Fisheries, Aquaculture and Irrigation, Szarvas 5540, Hungary 3: Centre for Environment, Fisheries and Aquaculture, Weymouth Laboratory, Weymouth Dorset DT4 8UB, U.K. 4: Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, U.K. 5: Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, K1N 6N5 Canada 6: Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, U.K. 7: Marine Environmental Biology, University of Southern California, Los Angeles, CA 90089, U.S.A.

Publication date: June 1, 2008

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