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A histological and morphometric study on the tissue and cellular distribution of iron in carp Cyprinus carpio L. during chronic waterborne exposure

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The effect of long-term (77 day) exposure of carp Cyprinus carpio to low concentrations of waterborne iron (1 mg Fe-dextran l−1) on accumulation and cellular distribution of iron in the liver was assessed using Perl’s staining and histological observations and morphometric measurements. Accumulation of iron in the liver occurred after 14 days of exposure, when the majority of the iron was found in the sinusoidal endothelium with lower amounts in the cytoplasm and nuclei of hepatocytes. Upon further treatment the iron was predominantly distributed in the cytoplasm of the hepatocytes either as granules or diffusive iron and in macrophages.
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Keywords: Perl’s reaction; carp; histology; iron; liver; morphometric measurements

Document Type: Research Article

Publication date: 2008-05-01

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