Microsatellite markers discriminate three species of North Atlantic wolffishes (Anarhichas spp.)

Authors: McCusker; Paterson; Bentzen

Source: Journal of Fish Biology, Volume 72, Number 2, February 2008 , pp. 375-385(11)

Publisher: Wiley-Blackwell

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Abstract:

Sixteen tetranucleotide and dinucleotide microsatellite markers were isolated from Atlantic wolffish, Anarhichas lupus, following a microsatellite enrichment procedure using probe-labelled magnetic beads. These microsatellites were intended for use in Atlantic wolffish as well as in two closely related species, spotted wolffish, Anarhichas minor, and northern wolffish, Anarhichas denticulatus. As all three species are of conservation concern in Canadian waters and as forensic wildlife cases may arise for this genus, microsatellite markers were assessed to determine how well they differentiate these species from one another and to estimate probability values that could be expected for identification of individuals to species.

Keywords: Anarhichas; marine fish; microsatellites; species identification; wolffish

Document Type: Regular Paper

DOI: http://dx.doi.org/10.1111/j.1095-8649.2007.01701.x

Affiliations: Department of Biology, Dalhousie University, 1355 Oxford Street, Halifax, Nova Scotia, B3H 4J1, Canada

Publication date: February 1, 2008

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