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Monoamine oxidase activity in kidney and heart of Piaractus mesopotamicus (Holmberg)

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Abstract:

The values of Michaelis–Menten constant (KM) and maximum velocity (VMAX) for kidney and heart monoamine oxidase (MAO) from pacu Piaractus mesopotamicus were determined. The mean ±s.e. KM values were 17·28 ± 2·27 M for kidney and 15·38 ± 1·86 M for heart. MAO activities were 111·60 ± 3·25 and 15·12 ± 0·30 nmols min−1 g−1 of wet tissue for kidney and heart, respectively. In addition, MAO inhibitory studies in these two tissues indicate that this enzyme may be a different isoform of MAO.

Keywords: MAO; clorgyline; deprenyl; heart; kidney

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1095-8649.2007.01637.x

Affiliations: 1: Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil 2: Departamento de Bioquímica, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

Publication date: December 1, 2007

bsc/jfb/2007/00000071/00000006/art00024
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