Optimization of fertilization success in Sander vitreus is influenced by the sperm : egg ratio and ova storage

Authors: Rinchard, J.1; Dabrowski, K.1; Van Tassell, J. J.2; Stein, R. A.2

Source: Journal of Fish Biology, Volume 67, Number 4, October 2005 , pp. 1157-1161(5)

Publisher: Wiley-Blackwell

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Abstract:

Walleye Sander vitreum eggs could be stored at 7° C for up to 30 h with 60% fertilization rates. Fertilization rates at 1 and 12° C fell to 17 and 5%, respectively. At spermatozoa : egg ratios <25 000 : 1, survival to the eyed stage declined precipitously. Beyond a ratio of 25 000 : 1, survival to the eyed stage did not increase >c. ∼ 70%. Therefore, a standard spermatozoa : egg ratio of 25 000 : 1 was established and is recommended for studies concerning fertilizing success.

Keywords: Sander vitreus; egg; fertilization; spermatozoa; walleye

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.0022-1112.2005.00800.x

Affiliations: 1: School of Natural Resources, The Ohio State University, 2021 Coffey Road, Columbus, OH 43210, U.S.A. and 2: Aquatic Ecology Laboratory, Department of Evolution, Ecology and Organismal Biology, The Ohio State University, 1314 Kinnear Road, Columbus, OH 43212, U.S.A.

Publication date: October 1, 2005

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