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Branchial release of free cortisol and melatonin by rainbow trout

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Individual rainbow trout Oncorhynchus mykiss were held in a specially constructed tank that enabled water to be collected separately from the anterior and posterior ends of the fish. Measurement by radioimmunoassay showed that >95% of the cortisol and melatonin released into the water originated from the anterior end (dominated by the gills). High performance liquid chromatography confirmed the identity of both hormones.

Keywords: cortisol; melatonin; non‐invasive assay; rainbow trout; stress

Document Type: Research Article


Affiliations: CEFAS Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset, DT4 8UB, U.K.

Publication date: 2005-08-01

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