The use of egg chorion glycoprotein of Epinephelus malabaricus for egg identification

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Abstract:

An immuno‐probe against a glycoprotein in the egg chorion was developed for egg identification. The 97 kD glycoprotein in the chorion of unfertilized eggs of Epinephelus malabaricus was isolated and separated by SDS‐PAGE as an antigen to induce antibody from rabbit. The reactivity of the antibody as the immuno‐probe to E. malabaricus eggs was significantly positive, and was specific in that it did not react with the eggs of other fish species. The immuno‐probe should be useful in identifying the eggs of E. malabaricus among mixed egg populations.

Keywords: Epinephelus malabaricus; chorion glycoprotein; egg identification; immuno‐probe

Document Type: Regular Paper

DOI: http://dx.doi.org/10.1111/j.0022-1112.2004.00571.x

Affiliations: 1: Institute of Marine Biology, National Taiwan Ocean University, 202 Keelung, Taiwan, 2: Institute of Botany, Academia Sinica, 11529 Taipei, Taiwan, 3: Department of Food Science, National Taiwan Ocean University, Taiwan and 4: Institute of Biotechnology, National Taiwan Ocean University, 202 Keelung, Taiwan

Publication date: December 1, 2004

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