Water‐based measurement of rainbow trout melatonin

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Melatonin (MLT) was isolated from water samples using solid phase extraction cartridges and measured by radioimmunoassay. In tanks of rainbow trout Oncorhynchus mykiss, concentrations of MLT in water increased rapidly from <0·1 ng l−1 in the light phase to 0·7 ng l−1 in the dark giving calculated release rates of <1 ng kg−1 h−1 and 15 ng kg−1 h−1, respectively. This cycle in water MLT values corresponds to reported changes in plasma MLT concentrations. The colour of the tanks and a daytime acute handling stress did not affect concentrations of MLT in either the light or dark phase.

Keywords: Oncorhynchus mykiss; melatonin; rainbow trout; water melatonin concentration

Document Type: Regular Paper

DOI: http://dx.doi.org/10.1111/j.0022-1112.2004.00531.x

Affiliations: CEFAS, Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, DT4 8UB, U.K.

Publication date: November 1, 2004

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