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Using the 13C and 15N of whitefish scales for retrospective ecological studies: changes in isotope signatures during the restoration of Lake Geneva, 1980–2001

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The scales of whitefish Coregonus lavaretus were used in place of dorsal muscle, which necessitates killing the fish, to study food webs from the 13C and 15N isotopic ratios in the organic fraction. As scales are composed of both organic and calcified fractions, a protocol for scale decalcification was first devised. The 13C and 15N values of the decalcified scales were then shown to be closely correlated to those of the dorsal muscle, demonstrating that scales could be used in place of muscle to study food webs. Changes in the 13C of whitefish were determined from a scale collection that extended over the period during which the trophic state of Lake Geneva was recovering.
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Keywords: fish scales; food web; trophic status; whitefish; 13C; 15N

Document Type: Regular Paper

Affiliations: Station d’Hydrobiologie Lacustre, INRA, 75, avenue de Corzent, BP 511, 74203 Thonon les Bains Cedex, France

Publication date: 2003-11-01

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