Skip to main content

Derivation of a pluripotent embryonic cell line from red sea bream blastulas

Buy Article:

$51.00 plus tax (Refund Policy)

Abstract:

A pluripotent cell line, sea bream embryonic stem‐like cells (SBES1), was developed from blastula‐stage embryos of the cultured red sea bream, Chrysophrys major. The SBES1 cells were cultivated in Dulbecco's modified eagles medium (DMEM) medium supplemented with foetal bovine serum, marine fish serum, fish embryo extract, selenium, basic fibroblast growth factor and leukemia inhibitory factor. They were small and round or polygonal, and grew actively and stabely in culture. The cells exhibited a positive alkaline phosphatase activity upon histochemical staining. When the cells were treated with all‐trans retinoic acid, they differentiated into various types including neuron‐like, neuroglia‐like and muscle‐like cells, suggesting that the SBES1 cells remained pluripotent in culture. Chromosome analysis revealed that SBES1 cells had a normal diploid karyotype with 2n = 2st + 46t. At present, SBES1 cells have been cultured for >180 days with more than 60 passages. High survival rate has been obtained after cryopreservation of cell cultures. This embryonic cell line may potentially be used for the production of transgenic red sea bream.

Keywords: Chrysophrys major; embryonic stem‐like cells; in vitro differentiation; pluripotency; red sea bream

Document Type: Regular Paper

DOI: http://dx.doi.org/10.1046/j.1095-8649.2003.00192.x

Affiliations: 1: Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Nanjing Road 106, Qingdao 266071, China, 2: Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260

Publication date: September 1, 2003

bsc/jfb/2003/00000063/00000003/art00017
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more