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Development and validation of methods for measuring multiple biochemical indices of condition in juvenile fishes

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The investigation established and validated methods to measure multiple biochemical indices of condition (whole body total lipids, whole body triglycerides, muscle RNA : DNA ratio and muscle protein) simultaneously in the same individual juvenile fish. It also provided examples of their application using a species comparison (salmonids and cyprinids) of the degree of change in these indices after food deprivation. The results showed that juvenile rainbow trout Oncorhynchus mykiss were much more susceptible to a decline in all biochemical indices of condition than juvenile fathead minnows Pimephales promelas upon food deprivation. The combination of these techniques can be used to accurately assess condition and growth potential of individuals from wild fish populations.

Keywords: RNA : DNA ratio; condition; food deprivation; lipid; triglyceride

Document Type: Regular Paper


Affiliations: 1: Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive Saskatoon, SK, S7N 5B4, Canada, 2: B.C. Hydro and Power Authority, 6911 Southpoint Drive (E16), Burnaby, BC V3N 4X8, Canada and 3: Department of Zoology, Oklahoma State University, Stillwater, OK 74078, U.S.A.

Publication date: 2003-09-01

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