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Assessment of digestive enzyme activities during larval development of white bream

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Activity of some of the main enzymes involved in protein digestion and absorption (acid and alkaline proteases, leucine-aminopeptidase, acid and alkaline phosphatases) as well as those of amylase and lipase, was assessed during larval development of white sea bream Diplodus sargus. All enzyme activity was detected at the moment of mouth opening. The variations observed in the activity profiles of the digestive enzymes were correlated either to developmental events, such as the functional start of the stomach (22 days after hatching), or to changes in the nature of the diet. The early and noticeable development of digestive enzyme activities was linked to a high survival after weaning.

Keywords: Diplodus sargus; SDS-PAGE; digestive enzymes; larval development

Document Type: Regular Paper


Affiliations: 1: Dpto. Biología Aplicada. E.P.S. Univ. Almería, E-04120 Almería, Spain, 2: CICEM El Toruño, Junta de Andalucía, P. O. Box 16, E-11500 El Puerto de Santa María, Cádiz, Spain and 3: Instituto de Ciencias Marinas de Andalucía (CSIC). Apartado Oficial, E-11510 Puerto Real, Cádiz, Spain

Publication date: July 1, 2003


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