Skip to main content

Molecular identification of Liriomyza trifolii (Burgess) (Dipt., Agromyzidae) based on real-time PCR

Buy Article:

$43.00 plus tax (Refund Policy)


Rapidly identifying juvenile individuals of Liriomyza trifolii (Burgess) from Liriomyza sativae Blanchard is crucial in plant quarantine. We report a molecular method to identify L. trifolii based on real-time polymerase chain reaction (PCR). By comparing partial DNA sequences of mitochondrial COI genes of L. trifolii samples collected from Guangdong and Taiwan provinces in China, Japan, Philippine, Israel, Germany, the USA, Mexico and Honduras sequenced by authors, and those of related species recorded in GenBank, a L. trifolii-specific probe was developed. There was no difference in individuals of different stages tested by this probe. The total time for real-time PCR assay system was 2 h, and it would save 3–7 h compared with conventional PCR.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: Liriomyza trifolii; molecular identification; mtDNA COI gene; real-time PCR

Document Type: Research Article

Affiliations: 1: Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, China 2: Guangdong Exit-Entry Inspection and Quarantine Bureau, Guangzhou, China

Publication date: 2007-09-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more