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Molecular identification of Liriomyza trifolii (Burgess) (Dipt., Agromyzidae) based on real-time PCR

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Abstract: 

Rapidly identifying juvenile individuals of Liriomyza trifolii (Burgess) from Liriomyza sativae Blanchard is crucial in plant quarantine. We report a molecular method to identify L. trifolii based on real-time polymerase chain reaction (PCR). By comparing partial DNA sequences of mitochondrial COI genes of L. trifolii samples collected from Guangdong and Taiwan provinces in China, Japan, Philippine, Israel, Germany, the USA, Mexico and Honduras sequenced by authors, and those of related species recorded in GenBank, a L. trifolii-specific probe was developed. There was no difference in individuals of different stages tested by this probe. The total time for real-time PCR assay system was 2 h, and it would save 3–7 h compared with conventional PCR.
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Keywords: Liriomyza trifolii; molecular identification; mtDNA COI gene; real-time PCR

Document Type: Research Article

Affiliations: 1: Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, China 2: Guangdong Exit-Entry Inspection and Quarantine Bureau, Guangzhou, China

Publication date: 2007-09-01

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