Microanalysis of propofol in human serum by semi-microcolumn high-performance liquid chromatography with UV detection and solid-phase extraction

Authors: Teshima D.; Nagahama H.; Makino K.; Kataoka Y.; Oishi R.

Source: Journal of Clinical Pharmacy & Therapeutics, Volume 26, Number 5, October 2001 , pp. 381-385(5)

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Abstract:

Objective: To develop a simple analytical method for monitoring the low serum levels of propofol found when administered for the sedation of patients in the intensive care unit (ICU).

Methods: A high-performance liquid chromatographic method (HPLC) was used with UV detection. Solid-phase extraction (SPE) cartridges and a semi-microcolumn (TSK gel ODS-80Ts, 2·0 mm i.d. × 25 cm, 5 m) were used to improve sensitivity. Propofol in the eluate obtained from the SPE cartridge was concentrated to about five times the initial concentration.

Results: The sensitivity using the semi-microcolumn was amplified by about three-fold. The assay showed a good linearity with a quantification limit 20 ng/mL. Intra- and inter-assay coefficients of variation were less than 2·2% and 10·0%, respectively. The mean recoveries ranged from 97·6 to 109·5%.

Conclusion: The HPLC method described should be useful for measuring the low serum propofol levels found when the drug is used for ICU sedation.

Keywords: ICU; propofol; sedation; semi-microcolumn

Language: English

Document Type: Miscellaneous

Publication date: 2001-10-01