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Geography determines genetic relationships between species of mountain pine (Pinus mugo complex) in western Europe

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Abstract Aim 

Our aims were to test whether morphological species of mountain pines were genetically supported in the western part of the distribution range of the Pinus mugo species complex (Pinus mugo Turra sensu lato), to resolve genetically homogeneous clusters of populations, to determine historical demographic processes, and to assess the potential hybridization of mountain pines with Scots pine, Pinus sylvestris L. Location 

Populations were sampled in the Iberian System, the Pyrenees, the French Mont Ventoux, Vosges and Jura mountains, the German Black Forest and throughout the Alps. This corresponded to a range-wide sampling for mountain pine sensu stricto (Pinus uncinata Ram.) and to a sampling of the western parts of the ranges of dwarf mountain pine (Pinus mugo Turra sensu stricto) and bog pine/peatbog pine [Pinus rotundata Link/Pinus×pseudopumilio (Willk.) Beck]. Methods 

In total, 786 individuals of P. mugo sensu lato from 29 natural populations, and 85 individuals of P. sylvestris from four natural populations were genotyped at three chloroplast microsatellites (cpSSRs). Populations were characterized for standard genetic diversity statistics and signs of demographic expansion. Genetic structure was explored using analysis of molecular variance, differentiation statistics and Bayesian analysis of population structure (BAPS). Results 

One hundred haplotypes were identified in P. mugo sensu lato. There was a stronger differentiation between geographical regions than between morphologically identified taxa (P. mugo sensu stricto, P. uncinata and P. rotundata/P. ×pseudopumilio). Overall genetic differentiation was weak (GST = 0.070) and displayed a clear phylogeographic structure [NST = 0.263, NST > NST (permuted), P< 0.001]. BAPS identified a Pyrenean and an Alpine gene pool, along with several smaller genetic clusters corresponding to peripheral populations. Main conclusions 

The core regions of the Pyrenees and Alps were probably recolonized, respectively by P. uncinata and P. uncinata/P. mugo sensu stricto, from multiple glacial refugia that were well connected by pollen flow within the mountain chains. Pinus rotundata/P. × pseudopumilio populations from the Black Forest, Vosges and Jura mountains were probably recolonized from various glacial populations that kept their genetic distinctiveness despite late glacial and early Holocene expansion. Marginal P. uncinata populations from the Iberian System are compatible with elevational shifts and long-term isolation. The causes of haplotype sharing between P. mugo sensu lato and P. sylvestris require further research.
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Keywords: Chloroplast microsatellites; Pinus mugo complex; Pinus sylvestris; conservation; genetic clusters; haplotype sharing; historical demography; hybridization; post-glacial recolonization; western Europe

Document Type: Research Article

Affiliations: 1: Department of Forest Systems and Resources, Centre of Forest Research CIFOR-INIA, Carretera de la Coruña km 7.5, 28040 Madrid, Spain 2: INRA, UR629, Ecologie des Forêts Méditerranéennes, Domaine St Paul, Site Agroparc, F-84914 Avignon, France 3: Consiglio Nazionale delle Ricerche, Istituto di Genetica Vegetale, Via Madonna del Piano 10, 50019 Sesto Fiorentino (Firenze), Italy

Publication date: 01 March 2010

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