IngentaConnect Investigation of the anti-fungal activity of coptisine on Candida...

Authors: Kong, W.-J.; Zhao, Y.-L.¹; Xiao, X.-H.¹; Li, Z.-L.²; Jin, C.¹; Li, H.-B.¹

Source: Journal of Applied Microbiology, Volume 107, Number 4, October 2009 , pp. 1072-1080(9)

Abstract:

Aims:

This study investigated the anti-fungal activity of coptisine on Candida albicans growth. Methods and Results:

The metabolic power-time curves of Candida albicans growth at 37°C affected by coptisine were measured by microcalorimetry using an LKB-2277 Bioactivity Monitor with stop-flow mode. Then, the diameter of inhibitory zones in the agar layer was observed using agar cup method, and the minimal inhibitory concentration (MIC) of coptisine on Candida albicans growth was determined by serial dilution method. From the principal component analysis on nine quantitative parameters obtained from the power-time curves, we could easily evaluate the anti-fungal activity of coptisine by analysing the change of values of the main two parameters, growth rate constant k and maximum power output in the log phase P_{m, log}. The results showed that coptisine had strong anti-fungal activity: at a low concentration (45 μg ml⁻¹) began to inhibit the growth of Candida albicans and at a high concentration (500 μg ml⁻¹) completely inhibited Candida albicans growth. Coptisine gave big inhibitory zones with diameters between 11 and 43 mm within test range, and the MIC of it was 1000 μg ml⁻¹. Conclusions:

Coptisine had strong anti-fungal activity on Candida albicans growth. The method of microcalorimetry applied for the assay of anti-fungal activity of coptisine was quantitative, sensitive and simple. Significance and Impact of the Study:

This work will provide useful information for the development of chemical biology policy in the use of anti-microbials in food and drug production.

Keywords: anti-fungal activity; Candida albicans; coptisine; microcalorimetry; principal component analysis

Document Type: Research article

DOI: 10.1111/j.1365-2672.2009.04292.x

Affiliations: 1: PLA Institute of Chinese Materia Medica, Hospital of People's Liberation Army, Beijing, China 2: Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu Sichuan, China

You have access to the full text article on a website external to Ingentaconnect.

Please click here to view this article on InterScience.

You may be required to register and activate access on InterScience before you can obtain the full text. If you have any queries please contact onlinehelp@oxon.blackwellpublishing.com

Investigation of the anti-fungal activity of coptisine on Candida albicans growth by microcalorimetry combined with principal component analysis