Authors: Simova, E.D.¹; Beshkova, D.B.¹; Dimitrov, Zh.P.²

Source: Journal of Applied Microbiology, Volume 106, Number 2, February 2009 , pp. 692-701(10)

Publisher: Wiley-Blackwell

Abstract:

Aims: 

To isolate bacteriocin-producing lactic acid bacteria (LAB) with high wide spectrum antibacterial activity and to characterize their inhibitory peptides. Method and Results: 

Seven LAB strains [Lactobacillus casei ssp. rhamnosus (PC5), Lactobacillus delbrueckii ssp. bulgaricus (BB18), Lactococcus lactis ssp. lactis (BCM5, BK15), Enterococcus faecium (MH3), Lactobacillus plantarum (BR12), Lactobacillus casei ssp. casei (BCZ2)], isolated from authentic Bulgarian dairy products were capable of producing bacteriocins, inhibiting the widest range of pathogenic bacteria. The bacteriocins were resistant to heating at 121°C for 15 min, stable at pH 2-10, sensitive to protease, insensitive to α-amylase and lipase. Two of bacteriocins produced by Lact. bulgaricus BB18 (bulgaricin BB18) and E. faecium MH3 (enterocin MH3) were purified and the molecular masses were determined. The N-terminal amino acid sequence of bulgaricin BB18 did not show strong homology to other known bacteriocins. Conclusions: 

Lactobacillus bulgaricus BB18 and E. faecium MH3 produce two novel bacteriocins highly similar to the pediocin-like nonlantibiotics. Significance and Impact of the Study: 

The two bacteriocins are potential antimicrobial agents and, in conjunction with their producers, may have use in applications to contribute a positive effect on the balance of intestinal microflora. Furthermore, bulgaricin BB18 strongly inhibits Helicobacter pylori.

Keywords: bacteriocin; Enterococcus; inhibitory spectrum; Lactobacillus; Lactococcus

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1365-2672.2008.04052.x

Affiliations: 1:  Laboratory of Applied Microbiology, Institute of Microbiology, Bulgarian Academy of Sciences, Plovdiv, Bulgaria 2:  LB `Bulgaricum' Research and Development Centre, Sofia, Bulgaria

Publication date: 2009-02-01

Related content

• In this: publication
• By this: publisher
• In this Subject: Microbiology
• By this author: Simova, E.D. ;  Beshkova, D.B. ;  Dimitrov, Zh.P.

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers