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Open Access Isolation of a homocysteine γ-lyase-producing bacterium and study of its enzyme production conditions

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Abstract Aim: 

To investigate the possibility of finding a new homocysteine (Hcy) γ-lyase with the desired properties for Hcy measurement in bacteria. Methods and Results: 

Through a process of enrichment, the Hcy γ-lyase-producing bacterium strain N2-1 was isolated from soil. Based upon its morphological, physiological, and biochemical characteristics, as well as its 16S rDNA sequence and phylogenetic tree analysis, this isolate belongs to the genus Serratia. The effects of pH, aeration, inducers, carbon (C) and nitrogen (N) sources on enzyme production were studied. Methionine, yeast extract, and glucose were selected as the optimal inducer, C and N sources, respectively. Maximum production of Hcy γ-lyase was obtained when the isolate was cultured at 30°C at pH 6·5 for about 36 h in the optimum medium. Results also showed that this Hcy γ-lyase has relatively high specificity towards Hcy. Conclusions: 

Because of its high specificity for Hcy, this bacterial Hcy γ-lyase has the potential application in Hcy determination. Significance and Impact of the Study: 

In addition to isolating a bacterium that produces Hcy γ-lyase suitable for Hcy determination, this study also indicates that the bacterium could be a source for production of Hcy γ-lyase for clinical applications.
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Keywords: Serratia; biotechnology; characterization; enzymatic assays; fermentation; homocysteine γ-lyase

Document Type: Research Article

Affiliations: 1:  College of Life Sciences, Zhejiang University, Hangzhou, China 2:  Zhejiang University City College, Hangzhou, China

Publication date: 01 April 2008

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