Fluorescent detection techniques for real-time multiplex strand specific detection of Bacillus anthracis using rapid PCR

Authors: Lee, M. A.; Brightwell, G.; Leslie, D.; Bird, H.; Hamilton, A.

Source: Journal of Applied Microbiology, Volume 87, Number 2, August 1999 , pp. 218-223(6)

Buy & download fulltext article:

You have access to the full text article on a website external to ingentaconnect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library

Abstract:

Speed is a key area in our development of PCR assays for Bacillus anthracis. We believe that the strand specific detection of amplicons within 10 min is a realistic goal and that this will be achieved through fluorescent in-tube assays. We have used the Idaho LightCycler^TM to study and develop candidate assays for B. anthracis. New strand specific fluorescent methods have been developed and a number of formats have been studied for speed and sensitivity. Internal controls have been developed as a method of improving our assay confidence. In this communication we will introduce the field of rapid PCR whilst discussing previous work in the areas described above, the development of our own rapid assay and a novel internal control system for B. anthracis. This work used PCR assays and hardware that are either commercially available, or have been previously described in open literature publications.

Document Type: Research article

DOI: http://dx.doi.org/10.1046/j.1365-2672.1999.00908.x

Affiliations: 1: DERA Porton Down, Salisbury, Wiltshire, UK

Publication date: 1999-08-01