Note: Sucrose transport and metabolism in Clostridium beijerinckii NCIMB 8052

Authors: Tangney; Rousse; Yazdanian; Mitchell

Source: Journal of Applied Microbiology, Volume 84, Number 5, June 1998 , pp. 914-919(6)

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Abstract:

Sucrose is the major carbon source in molasses, the traditional substrate employed in the industrial acetone-butanol-ethanol (ABE) fermentation by solventogenic clostridia. The utilization of sucrose by Clostridium beijerinckii NCIMB 8052 was investigated. Extracts prepared from cultures grown on sucrose (but not xylose or fructose) as the sole carbon source possessed sucrose phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) activity. Extract fractionation and reconstitution experiments revealed that the entire sucrose Enzyme II complex resides within the membrane in this organism. Sucrose-6-phosphate hydrolase and fructokinase activities were also detected in sucrose grown cultures. The fructokinase activity, which is required specifically during growth on sucrose, was shown to be inducible under these conditions. A pathway for sucrose metabolism in this organism is proposed.

Document Type: Research article

DOI: http://dx.doi.org/10.1046/j.1365-2672.1998.00432.x

Affiliations: 1: Department of Biological Sciences, Heriot-Watt University, Edinburgh, UK

Publication date: 1998-06-01