Expressed sequence tags and proteomics of antennae from the tortricid moth, Epiphyas postvittana

Authors: Jordan, M. D.; Stanley, D.; Marshall, S. D. G.; De Silva, D.; Crowhurst, R. N.; Gleave, A. P.; Greenwood, D. R.; Newcomb, R. D.

Source: Insect Molecular Biology, Volume 17, Number 4, August 2008 , pp. 361-373(13)

Publisher: Wiley-Blackwell

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Abstract:

Abstract

Genomic and proteomic analyses of the antennae of the light brown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae) were undertaken to identify genes and proteins potentially involved in odorant and pheromone binding and turnover. An EST approach yielded 5739 sequences, comprising 808 contigs and 1545 singletons. InterPro and Blastanalyses revealed members of families implicated in odorant and pheromone binding (PBPs, GOBPs, ABPXs and CSPs) and turnover (CXEs, GSTs, CYPs). Of the three pheromone binding proteins (PBPs) identified, two were more highly expressed at the RNA and protein levels in adult male antennae (EpPBP1, EpPBP3), while a third was more highly expressed in female antennae (EpPBP2). To identify proteins involved in the detection of sex-specific signals, differential 2D gel electrophoresis (pH 5–8) followed by mass spectrometry was conducted on antennal proteins from males versus females. Identified male-biased proteins included a pheromone binding protein, a porin, a short chain dehydrogenase/reductase, and a member of the takeout family.

Keywords: EST library; Epiphyas postvittana; Tortricidae; antenna; odorant binding proteins; odorant degrading enzymes; olfaction; proteomics and genomics

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1365-2583.2008.00812.x

Affiliations: The Horticultural and Food Research Institute of New Zealand Limited (HortResearch), Auckland, New Zealand;

Publication date: August 1, 2008

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