Transcriptional expression of survivin and its splice variants in cervical carcinomas

Authors: FUTAKUCHI¹; UEDA; KANDA¹; FUJINO¹; YAMAGUCHI¹; NODA²

Source: International Journal of Gynecological Cancer, Volume 17, Number 5, September/October 2007 , pp. 1092-1098(7)

Publisher: Wiley-Blackwell

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Abstract:

Futakuchi H, Ueda M, Kanda K, Fujino K, Yamaguchi H, Noda S. Transcriptional expression of survivin and its splice variants in cervical carcinomas. Int J Gynecol Cancer 2007;17:1092-1098.

The objective of this study was to evaluate transcriptional expression of survivin and the two splice variants (survivin-2B and survivin-ΔEx3) in cervical carcinomas. The gene expression levels of survivin and its splice variants in 11 human cervical carcinoma cell lines and 20 malignant and 12 normal cervical tissue samples were analyzed using quantitative reverse transcription-polymerase chain reaction analysis. Gene expression levels of survivin and survivin-ΔEx3 in cell lines were higher than those in normal cervical tissues (P= 0.0193 and 0.0489). Transcript levels of survivin and survivin-ΔEx3 in carcinoma tissues were also higher than those in normal controls (P= 0.0016 and 0.0011). Gene expression levels of survivin and survivin-ΔEx3 in adenocarcinomas were statistically higher than those in squamous cell carcinomas (P= 0.0260 and 0.0487). There was no significant difference in survivin-2B gene expression between malignant and normal cervical samples or different histologic types. The ratios of survivin-2B/survivin and survivin-ΔEx3/survivin in carcinoma tissues were higher than those in normal controls (P= 0.0288 and 0.0081). Interestingly, the ratio of survivin-2B/survivin was increased in the patients with higher stages and with pelvic lymph node metastasis (P= 0.0205 and 0.0437), respectively. We conclude that survivin and its splice variants might be involved in the pathogenesis and development of cervical carcinomas.

Keywords: apoptosis; cervical carcinoma; splice variants; survivin

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1525-1438.2007.00833.x

Affiliations: 1: Department of Obstetrics and Gynecology, Osaka Medical College, Osaka, Japan 2: Cytopathology and Gynecology, Osaka Cancer Prevention and Detection Center, Osaka, Japan

Publication date: 2007-09-01

Transcriptional expression of survivin and its splice variants in cervical carcinomas

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