Authors: Nakayama A.¹; Ogiso B.; Tanabe N.¹; Takeichi O.; Matsuzaka K.²; Inoue T.²

Source: International Endodontic Journal, Volume 38, Number 4, April 2005 , pp. 203-210(8)

Publisher: Wiley-Blackwell

Abstract:

Nakayama A, Ogiso B, Tanabe N, Takeichi O, Matsuzaka K, Inoue T. Behaviour of bone marrow osteoblast-like cells on mineral trioxide aggregate: morphology and expression of type I collagen and bone-related protein mRNAs. International Endodontic Journal, 38, 203–210, 2005. Abstract Aim

To investigate the in vitro behaviour of rat bone marrow cells (RBM) on mineral trioxide aggregate (MTA) (ProRoot^TM, MTA Root Canal Repair Material; Dentsply Tulsa, Tulsa, OK, USA) compared with intermediate restorative materials (IRM) (Dentsply Caulk, Milford, DE, USA). Methodology

RBM were obtained from rat femur and were primary cultured and then subcultured. Cells were then seeded on three dishes of each material, and cultured for 3 days, after which they were evaluated morphologically using scanning (SEM) and transmission (TEM) electron microscopy. Furthermore, the calcium released from hydrated material, the cell proliferation ratio and alkaline phosphatase (ALP) activity were analysed, and the expression of type I collagen and bone-related protein mRNAs were evaluated. The data were averaged and analysed via one-way analysis of variance (anova) and were then compared by the Scheffe's test. Results

SEM showed that RBM attached to MTA and had a flattened appearance without nuclear protrusions and microspikes. TEM showed that the cells attached in the same manner as the control group, but gaps larger than 2 m were frequently seen. The calcium released from hydrated MTA was about 130 ppm after 3 days of immersion in saline. The ALP activity was similar to the control group. Cell proliferation and expression of type I collagen mRNA was significantly lower, while the expression of osteopontin mRNA was significantly higher than the control group at the third day of culture. In IRM groups, a few rounded cells were observed on the material but no living cells were seen. Conclusions

MTA is a material of low toxicity which does not inhibit cell growth, but does suppress the differentiation of osteoblast-like cells.

Keywords: intermediate restorative material; mineral trioxide aggregate; osteoblast-like cells; rat

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1365-2591.2004.00917.x

Affiliations: 1: Department of Endodontics, Nihon University School of Dentistry, Tokyo, Japan 2: Department of Clinical Pathophysiology, Oral Health Science Center, Tokyo Dental College, Chiba, Japan

Publication date: 2005-04-01

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