IngentaConnect The Use of an Oral Fluid Immunoglobulin G ELISA for the Detection...

Authors: Gilger M.A.; Tolia V.¹; Johnson A.²; Rabinowitz S.³; Jibaly R.³; Elitsur Y.⁴; Chong S.⁵; Rosenberg A.⁶; Gold B.⁷; Rosenthal P.⁸; Elkayam O.⁸; Marchildon P.⁹; Peacock J.⁹

Source: Helicobacter, Volume 7, Number 2, April 2002 , pp. 105-110(6)

Publisher: Blackwell Publishing

Abstract:

Background.

Enzyme linked immunosorbent assay (ELISA) evaluation of oral fluid immunoglobulin G (IgG) antibodies to Helicobacter pylori is a unique approach for both epidemiological studies and the diagnosis of infection, especially in children. The use of oral fluid sampling to evaluate specific H. pylori IgG antibodies has advantages over serum, including reduced biohazard risk and noninvasive collection. Oral fluid sampling is fast and involves minimal patient discomfort. Since children facilitate transmission of H. pylori infection, a simple, accurate, noninvasive diagnostic test is necessary for large epidemiologic studies. The aim of our study was to evaluate a new oral fluid ELISA for detection of IgG antibodies to H. pylori in children. Materials and methods.

We compared this new oral fluid ELISA with the HM-CAP^TM serum ELISA and gastric biopsy histology using 779 oral fluid samples from children collected at 11 clinical sites across the United States. This cohort included 315 children symptomatic for abdominal pain and 464 asymptomatic. All samples were evaluated in a double blind manner. The oral fluid ELISA demonstrated a sensitivity of 76.2% and a specificity of 94.0% in children 2 months old to 20¹/₂ years, as compared with the HM-CAP^TM serologic assay. The assay’s sensitivity improved to 81.3% in children aged 5 or greater and the specificity remained at 94.0%. When compared with gastric biopsy histology in the same age group, the oral fluid ELISA demonstrated a sensitivity of 71.7% and a specificity of 90.4%. Results.

This new oral fluid ELISA is moderately sensitive and offers a very specific method for detecting H. pylori infection in older children, but it is of little value in children under the age of 5 years. Conclusions.

Overall, we conclude that this oral fluid ELISA does not appear to be a helpful clinical tool for the diagnosis of H. pylori infection in children.

Keywords: Peptic ulcer disease in children; gastric infection in children; salivary ELISA

Document Type: Research article

DOI: 10.1046/j.1083-4389.2002.00062.x

Affiliations: 1: Children’s Hospital of Michigan, Detroit, MI, USA; 2: Texas Tech University Health Sciences Center, Amarillo, TX, USA; 3: SUNY-Health Science Center, Brooklyn, NY, USA; 4: Marshall University School of Medicine, Huntington, WV, USA; 5: Queen Mary’s Hospital for Children, Surrey, UK; 6: East Carolina University of Medicine, Greenville, NC, USA; 7: Emory University School of Medicine, Atlanta, GA, USA; 8: University of California San Francisco, San Francisco, CA, USA; 9: Enteric Products, Inc, Stony Brook, NY, USA

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$50.16 plus tax Refund Policy

The Use of an Oral Fluid Immunoglobulin G ELISA for the Detection of Helicobacter pylori Infection in Children