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Combinations of lactic acid bacteria and yeast suitable for preparation of marine silage

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 Marine silage (MS) is a fish hatchery diet prepared by decomposing seaweed into cellular units and performing lactic acid fermentation. MS was first developed by using a wild-type consortium composed of a lactic acid bacterium (LAB) Lactobacillus brevis and yeasts Debaryomyces hansenii var. hansenii and Candida sp. as a fermentation starter. To further examine a suitable combination of starter microorganisms, MS was prepared with different compositions of LAB and yeasts from a substrate of Undaria pinnatifida. Production efficiency of algal single cell units was not different among the trials. Inoculation of LAB significantly retarded the growth of contaminant microorganisms while a single inoculation of yeasts could not retard the growth of halophilic contaminant microorganisms. Lactobacillus brevis showed the highest predominating ability in MS among the tested, while L. acidophilus (casei-type) IAM 10074 and L. plantarum IAM12477T also showed predominating ability with relatively inferior scores when analyzed from the results of the identification with 16S rRNA-targeted species-specific primers. The present study demonstrated that a single use of LAB, including food industry-familiar species, is suitable for preparation of MS, while the superiority of L. brevis B5201 was suggested in predominating ability in marine silage.

Keywords: 16S rRNA-targeted species-specific primer; lactic acid bacteria; marine silage; starter; yeast

Document Type: Research Article


Affiliations: 1: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Ohno, Hiroshima 739-0452 and 2: National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, Kanagawa 236-8648, Japan

Publication date: 2004-06-01

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