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Genetic variation detected with random amplified polymorphicDNA markers among isolates of the red rot disease fungus Pythiumporphyrae isolated from Porphyra yezoensis from Koreaand Japan

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Genetic variation of the fungal parasite Pythium porphyrae,causative organism of red rot disease of Porphyra, isolatedfrom Asan, Mokpo, Pusan and Wando in Korea, and from Aichi, Fukuokaand Miyagi in Japan was investigated by random amplified polymorphicDNA (RAPD) cluster analysis. The total 67 RAPD markers were generatedfrom 38 isolates by RAPD-polymerase chain reaction (PCR) using arbitraryprimers consisting of 10 nucleotide sequences and 33 of them indicatedpolymorphisms. The dissimilarity coefficients calculated from theRAPD banding patterns ranged from 0.0010 to 0.6983. The dendrogramgenerated by the unweighted pair-group method using arithmetic averagesshowed that the 38 isolates were classified into three clusters(Groups 1, 2 and 3). Group 1 consisting of two isolates from Miyagiwas separated from all other isolates by a genetic distance of 0.6983.Groups 2 and 3 containing the majority of the isolates were branchedon genetic distance of 0.3957. These two clusters subdivided intofour and three subclusters, respectively, which were apparentlyassociated with geographic origins of the isolates. Interestingly,the isolates from Asan of Korea were close to Japanese isolatesrather than Korean isolates on genetic diversity. In addition, thegenetic distances of intra-isolates from Japan were higher thanthose from Korea.

Keywords: Pythium porphyrae; fungus; genetic variation; randomamplified polymorphic DNA-polymerase chain reaction (RAPD-PCR); red rot disease

Document Type: Research Article


Affiliations: Laboratory of Marine Biochemistry,Faculty of Bioresources, Mie University, Tsu, Mie 514-8507, Japan

Publication date: April 1, 2003

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