Role of prostaglandin in the control of ovulation in the Japaneseeel Anguilla japonica

Authors: * H.K.¹; TANAKA H.¹; UNUMA T.¹; † H.O.; GEN K.²; OKUZAWA K.²

Source: Fisheries Science, Volume 69, Number 2, April 2003 , pp. 234-241(8)

Publisher: Wiley-Blackwell

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Abstract:

The in vitro effects of 17,20 bgr

-dihydroxy-4-pregnen-3-one(DHP) and prostaglandins (PGE₁, PGE₂, PGF₁,PGF₂) on ovulation in the Japanese eel Anguillajaponica were examined. Oocytes with follicle layers at themigratory nucleus stage (approximately 850–900 µmdiameter) were removed using a polyethylene cannula from artificiallymatured fish. At concentrations of 10 and 100 ng/mL,DHP was found to induce both germinal vesicle breakdown and ovulation.The prostaglandins, except for PGE₁, effectively inducedovulation of previously matured oocytes by DHP treatment in vitro.Prostaglandin F₂ was the most effective. Asignificant increase in ovulation rate was observed even at a concentrationof 0.01 µg/mL PGF₂.Indomethacin blocked the in vitro ovulation induced by DHPand addition of PGF₂ reversed indomethacin-blockedovulation. Actinomycin D and cycloheximide blocked DHP-induced ovulationand PGF₂ reversed the effects of both inhibitors. Theseresults indicate that DHP induces ovulation through endogenous prostaglandinsynthesis in the follicle layers of the Japanese eel.

Keywords: actinomycin D; cycloheximide; in vitro ovulation; indomethacin; Japanese eel; prostaglandin

Document Type: Research article

DOI: http://dx.doi.org/10.1046/j.1444-2906.2003.00613.x

Affiliations: 1: National Research Instituteof Aquaculture, Fisheries Research Agency, Nansei, Mie 516-0193and 2: Inland Station, National ResearchInstitute of Aquaculture, Fisheries Research Agency, Tamaki, Mie 519-0423, Japan

Publication date: 2003-04-01