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Role of prostaglandin in the control of ovulation in the Japaneseeel Anguilla japonica

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ABSTRACT: 

The in vitro effects of 17,20β-dihydroxy-4-pregnen-3-one(DHP) and prostaglandins (PGE1, PGE2, PGF,PGF) on ovulation in the Japanese eel Anguillajaponica were examined. Oocytes with follicle layers at themigratory nucleus stage (approximately 850–900 µmdiameter) were removed using a polyethylene cannula from artificiallymatured fish. At concentrations of 10 and 100 ng/mL,DHP was found to induce both germinal vesicle breakdown and ovulation.The prostaglandins, except for PGE1, effectively inducedovulation of previously matured oocytes by DHP treatment in vitro.Prostaglandin F was the most effective. Asignificant increase in ovulation rate was observed even at a concentrationof 0.01 µg/mL PGF.Indomethacin blocked the in vitro ovulation induced by DHPand addition of PGF reversed indomethacin-blockedovulation. Actinomycin D and cycloheximide blocked DHP-induced ovulationand PGF reversed the effects of both inhibitors. Theseresults indicate that DHP induces ovulation through endogenous prostaglandinsynthesis in the follicle layers of the Japanese eel.
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Keywords: Japanese eel; actinomycin D; cycloheximide; in vitro ovulation; indomethacin; prostaglandin

Document Type: Research Article

Affiliations: 1: National Research Instituteof Aquaculture, Fisheries Research Agency, Nansei, Mie 516-0193and 2: Inland Station, National ResearchInstitute of Aquaculture, Fisheries Research Agency, Tamaki, Mie 519-0423, Japan

Publication date: 2003-04-01

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